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(C) Comparison of high resolution collision induced dissociation (CID) mass spectra of the metabolite m/z 265.1188 in plasma (red) and synthetic PAGln standard (blue). (D) In the Validation Cohort (n = 4,000) and indicated sub cohorts, Kaplan Meier estimates and the risk of MACE according to PAGln quartile levels as measure by stable isotope

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For further validation of the model, leave one out cross validation was performed (Fig. 2ce). Sensitivity is the parameter that measures the ability of the model to correctly classify cases, whereas specificity measures the predictive ability of the model to correctly classify the controls ((Szymanska et al., 2012)).

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